PSVI-2 Relationship of Sperm Plasma Serine Protease Inhibitor (Serpina5) and Dystroglycan (DAG1) Proteins with Dairy Bull Fertility

Abstract It has been reported that plasma serine protease inhibitor (SERPINA5) and dystroglycan (DAG1) are loosely attached to the sperm SERPINA5 can also be present on tails. The objective of this study was evaluate whether and/or DAG1 could used as a fertility marker in dairy bulls. Frozen semen from bulls (n=19) were evaluated for concentration percentage tail labeled (SERPINA5-tail) by immunofluorescence minimum 200 cells per bull two different ejaculates. Semen total motility, progressive viability. vitro embryo production (~150 oocytes/bull). Bull classified methods, sire conception rates [SCR - High-SCR ( >1.0) or Low-SCR (< -4.0)] ratio embryos developed into blastocyst (BL) those had cleaved (CL) Good (BL/CL≥38.5%) Poor (BL/CL< 38.5%). GLIMMIX procedure SAS classification with SCR, BL/CL, interaction model random effect. Progressive CL, concentration, SERPINA5-tail did not differ (P≥0.14) among SCR BL/CL classifications their interaction. There no difference (P>0.33) motility between classification; however, significant (P=0.02; High-SCR/Good 54.9±2.8%, High-SCR/Poor 39.4±5.4%, Low-SCR/Good 44.8±4.5%, Low-SCR/Poor 52.03±3.2%). decreased BL (P=0.03; 30.6±1.6%, 35.8±1.4%, respectively) tended have (P=0.10; 38.1±2.0%, 42.9±1.8%, compared In summary, do seem putative marker. is good predictor since may developments.